Recombinant Histone 3 anticorps (H3K27me3)

N° du produit ABIN7072680

Cet anticorps anti-Histone 3 est un anticorps Lapin Chimeric détectant Histone 3 dans WB, IHC, IF, ELISA, IP et ICC. Adapté pour Humain.

Aperçu rapide pour Recombinant Histone 3 anticorps (H3K27me3) (ABIN7072680)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BT164

Détail du produit anti-Histone 3 anticorps

Épitope: H3K27me3

Fonction: Anti-H3K27me3 [BT164], Rabbit IgG, kappa

Specificité: The epitope of BT164 was mapped in the N-terminal tail of histone H3 (27-KSAPAT-32) and included the apoptosis-induced trimethylation of K27.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: BT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin.

Isotype: IgG kappa

Information d'application

Indications d'application: BT164 recognizes an apoptosis-induced trimethylation of K27 on N-terminal tail of histone H3 which is a target for autoantibodies in patients and mice with SLE (systemic lupus erythematosus) (van Bavel et al., 2011, pmid: 20699234). Consequently, this antibody is recommended for the detection and studying of SLE. ELISA studies demonstrated that BT164 exhibits preferential activity with the apoptotic nucleosomes in comparison with the control nucleosomes (van Bavel et al., 2011, pmid: 20699234). It can be used for the detection of apoptosis in general. Furthermore, this antibody was demonstrated to label cells with methylated histone-3 on K27 via immunofluorescence and it also immunoprecipitated chromatin with such a methylation profile, further demonstrating its ability to specifically recognize markers characteristic of SLE (van Bavel et al., 2011, pmid: 20699234).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Trimethyl-Histone H3 (Lys27), histone H3, H3K27, Trimethylated histone H3, Histone H3 trimethylated at Lysine 27, nucleosome, H3K27ME3, post translational modification, histone post-translational modification