Recombinant CD40 anticorps (Extracellular Domain)

N° du produit ABIN7072685

L’anticorps Lapin Chimeric anti-CD40 a été validé pour BR. Il convient pour détecter CD40 dans des échantillons de Humain.

Aperçu rapide pour Recombinant CD40 anticorps (Extracellular Domain) (ABIN7072685)

Antigène: CD40

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Blocking Reagent (BR)

Clone: Chi220

Détail du produit anti-CD40 anticorps

Épitope: Extracellular Domain

Fonction: Anti-CD40 [chi220], Rabbit IgG, kappa

Specificité: This antibody binds the CD40 and blocks the humoral immune responses to T cell dependent antigens in a dose dependent manner.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody blocks the interaction between CD40 and gp39. Chi220 was used either alone or in combination with CTLA4-Ig in an immunosuppresive therapy study to check the survival of renal allografts in non human primate models (PMID: 12394833). Chi220 blocks binding of CD154, it also has partial agonist properties and weak stimulatory potential. It synergisticaly works with LEA29Y (belatacept) to prolong the islet allograft survival in a non-human primate Macaca fascicularis (PMID: 15611281). A study on protection of islets xenographs in diabetic macaques used a combination of Chi220 with IL-2 receptor specific antibody Basiliximab for immunosuppression with induction therapy. Chi220 is used as an sCD154 blocking agent and it was seen that it binds sCD154 in a dose dependant manner (PMID: 21920020). Two humanized versions of this antibody were generated F4 and L3.17, also showed similar properties. Humanized versions of this antibody are available on request.

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD40

Antigène: CD40

Autre désignation: CD40

Sujet: TNFSF5, CDw40, TRAP, p50, Bp50, CD40L receptor, Tumor necrosis factor ligand superfamily member 5, B-cell surface antigen CD40, T-cell antigen Gp39, TNF-related activation protein.

UniProt: P29965

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints