Recombinant GM2 Ganglioside anticorps

N° du produit ABIN7072745

L’anticorps anti-GM2 Ganglioside Monoclonal Human est utilisé pour la détection de GM2 Ganglioside dans des échantillons de Humain. Il a été validé pour ELISA, IHC, Dep et TLC Overlay.

Aperçu rapide pour Recombinant GM2 Ganglioside anticorps (ABIN7072745)

Antigène: GM2 Ganglioside

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Human

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Immunohistochemistry (IHC), Depletion (Dep), Thin Layer Chromatography Overlay (TLC Overlay)

Clone: L55-81

Détail du produit

Fonction: Anti-GM2 ganglioside [L55-81], Human IgM, kappa

Specificité: L55-81 binds specifically to GM2 ganglioside, this antibody shows no reactivity towards GM1, GM3, GD2 or normal tissues- the L55-81 epitope contains both a GalNAc and sialic acid bound to lactose. Ganglioside GM2 (GalNAcβ1-4(NeuNAcα2-3)Galβ1-4Glcβ-1ceramide) is a glycosphingolipid present on the cell surface and a major tumor-differentiation antigen in human melanoma and hepatoma.

Attributs du produit:

Original Species of Ab: Human

Original Format of Ab: IgM

Purification: Affinity Purified using a recombinant lectin column

Immunogène: L55-81 was prepared by isolating EBV-immortalized peripheral blood B lymphocytes from a human donor with melanoma.

Isotype: IgM kappa

Information d'application

Indications d'application: L55-81 reacts positively to melanoma cell lines with high GM2 expression (M14, M24), and shows no binding to normal cell types or cell lines M15 or M25 which have low GM2 expression and high GM3 expression. This specificity can be confirmed by ELISA and TLC immunostaining by testing a variety of gangliosides and neutral glycolipids. L55-81 is able to lyse cells from the M14 melanoma cell line (which has high GM2 expression) in the presence of human or rabbit complement, but not in the absence of complement - this was shown by a [51Cr] release assay. Biotinylated L55-81 has been used for IHC of acetone-fixed sections of tumor tissue in an avidin-biotin complex immunoperoxidase system. Statistically significant positive staining (determined by Fisher's exact test) was displayed with melanoma, colon carcinoma and ovary carcinoma tumor tissues. Staining has also be observed with breast carcinoma, prostate carcinoma and kidney carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: GM2 Ganglioside