Recombinant GM2 Ganglioside anticorps

N° du produit ABIN7543165

Cet anticorps anti-GM2 Ganglioside Chimeric Lapin (Clone KM966) (ABIN7543165) détecte spécifiquement GM2 Ganglioside dans ELISA, IF, FACS et ICC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant GM2 Ganglioside anticorps (ABIN7543165)

Antigène: GM2 Ganglioside

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Inconjugué

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: KM966

Détail du produit

Fonction: Anti-GM2 ganglioside [KM966], Rabbit IgG, kappa

Specificité: This antibody recognizes and binds ganglioside GM2.

Attributs du produit:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: This chimeric antibody was generated by cloning the variable heavy and light chain sequences of murine parent antibody KM696 (IgM) and expressing it as a mouse/human IgG1. The parent antibody was raised against human GM2.

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody shows antitumor activity both in vitro and in vivo. Chimeric KM966 markedly suppressed the establishment of f human tumor xenografts in nude mice. Indirect immunofluorescence staining of tumor cell lines with the chimeric KM966 revealed that the antigen was expressed in substantial amounts on pulmonary tumor cells and leukemia cells as well as neuroectodermal origin tumor cells. This antibody also reacts with GM2 in an ELISA and also can be used for the identification of tumor cells expressing GM2 via flow cytometry (PMID: 8137257). The effect of chimeric antibody KM966 on GM2 expressing human solid tumors in vivo was done in PMID: 10417777. This antibody was also used in the immunocytochemical staining of cultured skin fibroblast of patients with GM2-gangliosidosis (PMID: 28417072). Humanized versions of this antibody KM8966, KM8967, KM8969 and KM8970 are available on request.

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: GM2 Ganglioside

Sujet: GM2