MSH2 anticorps

N° du produit ABIN7091416

L’anticorps Lapin Monoclonal anti-MSH2 a été validé pour WB, IF, ICC et IHC (p). Il convient pour détecter MSH2 dans des échantillons de Humain.

Aperçu rapide pour MSH2 anticorps (ABIN7091416)

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: B4F3

Détail du produit anti-MSH2 anticorps

Réactivité croisée: Humain

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human MSH2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IF()
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur MSH2

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Autre désignation: MSH2

Sujet:

Synonyms: MSH-2, BAT26, DNA mismatch repair protein Msh2, FCC1, COCA1, HNPCC, LCFS2, HNPCC1, BAT26, COCA 1, COCA1, DNA mismatch repair protein Msh2, FCC 1, FCC1, hMSH2, HNPCC 1, HNPCC, HNPCC1, LCFS2, MSH 2, Msh2, MSH2_HUMAN, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2.

Background: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

ID gène: 4436

UniProt: P43246

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response