CHFR anticorps

N° du produit ABIN7112653

Cet anticorps Lapin Polyclonal détecte spécifiquement CHFR dans ELISA, WB et IHC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour CHFR anticorps (ABIN7112653)

Antigène: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHFR est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-CHFR anticorps

Fonction: CHFR antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: checkpoint with forkhead and ring finger domains

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur CHFR

Antigène: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Autre désignation: CHFR

Sujet:

Synonyms: E3 ubiquitin-protein ligase CHFR|Checkpoint with forkhead and RING finger domains protein|RING finger protein 196|RING-type E3 ubiquitin transferase CHFR|CHFR|RNF196

Background: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2(UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Poids moléculaire: 70-85 kDa

ID gène: 55743

UniProt: Q96EP1