DNA2 anticorps

N° du produit ABIN7113557

L’anticorps Lapin Polyclonal anti-DNA2 a été validé pour ELISA, IHC, WB et IF. Il convient pour détecter DNA2 dans des échantillons de Humain et Souris.

Aperçu rapide pour DNA2 anticorps (ABIN7113557)

Antigène: DNA2 (DNA Replication Helicase 2 Homolog (DNA2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNA2 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-DNA2 anticorps

Fonction: DNA2 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: DNA replication helicase 2 homolog

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:1000, IHC: 1:50-1:500, IF: 1:10-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur DNA2

Antigène: DNA2 (DNA Replication Helicase 2 Homolog (DNA2))

Autre désignation: DNA2

Sujet:

Synonyms: DNA replication ATP-dependent helicase/nuclease DNA2 (hDNA2)|DNA replication ATP-dependent helicase-like homolog|DNA2|DNA2L|KIAA0083

Background: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Poids moléculaire: 127 kDa

ID gène: 1763

UniProt: P51530

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA