LIN28B anticorps

N° du produit ABIN7115882

L’anticorps Lapin Polyclonal anti-LIN28B a été validé pour WB et ELISA. Il convient pour détecter LIN28B dans des échantillons de Humain et Souris.

Aperçu rapide pour LIN28B anticorps (ABIN7115882)

Antigène: LIN28B (Lin-28 Homolog B (LIN28B))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIN28B est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-LIN28B anticorps

Fonction: LIN28B antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène:

Immunogen sequence: 5-250AA

Immunogen: lin-28 homolog B

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur LIN28B

Antigène: LIN28B (Lin-28 Homolog B (LIN28B))

Autre désignation: LIN28B

Sujet:

Synonyms: Protein lin-28 homolog B (Lin-28B)|LIN28B|CSDD2

Background: Suppressor of microRNA(miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts(pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA(PubMed:22118463). Does not act on pri-miR21(PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation(PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent(PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression(By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Poids moléculaire: 27 kDa

ID gène: 389421

UniProt: Q6ZN17