MSH2 anticorps (AA 585-934)

N° du produit ABIN7116323

L’anticorps Souris Monoclonal anti-MSH2 a été validé pour WB, ELISA et IHC. Il convient pour détecter MSH2 dans des échantillons de Humain.

Aperçu rapide pour MSH2 anticorps (AA 585-934) (ABIN7116323)

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 7C1

Détail du produit anti-MSH2 anticorps

Épitope: AA 585-934

Fonction: MSH2 antibody

Purification: Protein A+G purification

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène:

Immunogen sequence: 585-934aa

Immunogen: mutS homolog 2, colon cancer, nonpolyposis type 1

Isotype: IgG2a

Information d'application

Indications d'application: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur MSH2

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Autre désignation: MSH2

Sujet:

Synonyms: DNA mismatch repair protein Msh2 (hMSH2)|MutS protein homolog 2|MSH2

Background: Component of the post-replicative DNA mismatch repair system(MMR). Forms two different heterodimers: MutS alpha(MSH2-MSH6 heterodimer) and MutS beta(MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Poids moléculaire: 105 kDa

ID gène: 4436

UniProt: P43246

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response