MLH1 anticorps

N° du produit ABIN7116540

Cet anticorps anti-MLH1 est un anticorps Lapin Polyclonal détectant MLH1 dans WB, ELISA, IHC et IP. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour MLH1 anticorps (ABIN7116540)

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MLH1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-MLH1 anticorps

Fonction: MLH1 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: MutL protein homolog 1

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:50-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MLH1

Sujet:

Synonyms: DNA mismatch repair protein Mlh1|MutL protein homolog 1|MLH1|COCA2

Background: Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system(MMR). DNA repair is initiated by MutS alpha(MSH2-MSH6) or MutS beta(MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha(MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Poids moléculaire: 85 kDa

ID gène: 4292

UniProt: P40692

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response