RPA1 anticorps

N° du produit ABIN7118402

L’anticorps Lapin Polyclonal anti-RPA1 a été validé pour WB et ELISA. Il convient pour détecter RPA1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour RPA1 anticorps (ABIN7118402)

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPA1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-RPA1 anticorps

Fonction: RPA1 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: replication protein A1, 70kDa

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500 - 1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur RPA1

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Autre désignation: RPA1

Sujet:

Synonyms: Replication protein A 70 kDa DNA-binding subunit (RP-A p70)|Replication factor A protein 1 (RF-A protein 1)|Single-stranded DNA-binding protein|Replication protein A 70 kDa DNA-binding subunit, N-terminally processed|RPA1|REPA1|RPA70

Background: As part of the heterotrimeric replication protein A complex(RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage(PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response(PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage(PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair(PubMed:7697716). Plays also a role in base excision repair(BER) probably through interaction with UNG(PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance(PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C(RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange(PubMed:19996105).

Poids moléculaire: 70 kDa

ID gène: 6117

UniProt: P27694

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA