USP13 anticorps

N° du produit ABIN7120497

Cet anticorps anti-USP13 est un anticorps Souris Monoclonal détectant USP13 dans WB, IHC, ELISA et IF. Adapté pour Humain.

Aperçu rapide pour USP13 anticorps (ABIN7120497)

Antigène: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp USP13 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 9E6

Détail du produit anti-USP13 anticorps

Fonction: USP13 antibody

Purification: Protein A+G purification

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: ubiquitin specific peptidase 13(isopeptidase T-3)

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:5000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur USP13

Antigène: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Autre désignation: USP13

Sujet:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 13|Deubiquitinating enzyme 13|Isopeptidase T-3 (ISOT-3)|Ubiquitin thioesterase 13|Ubiquitin-specific-processing protease 13|USP13|ISOT3

Background: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation(ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation(ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro, however, additional experiments are required to confirm such data.

Poids moléculaire: 97 kDa

ID gène: 8975

UniProt: Q92995

Pathways: SARS-CoV-2 Protein Interactome