USP28 anticorps

N° du produit ABIN7120510

Cet anticorps Lapin Polyclonal détecte spécifiquement USP28 dans WB, ELISA, IHC et IP. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour USP28 anticorps (ABIN7120510)

Antigène: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp USP28 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-USP28 anticorps

Fonction: USP28 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: ubiquitin specific peptidase 28

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:5000, IP: 1:200-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur USP28

Antigène: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Autre désignation: USP28

Sujet:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 28|Deubiquitinating enzyme 28|Ubiquitin thioesterase 28|Ubiquitin-specific-processing protease 28|USP28|KIAA1515

Background: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome(APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7(FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7(FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes(TSGs) in a subset of colorectal cancers(CRC) cells(PubMed:24623306).

Poids moléculaire: 130 kDa

ID gène: 57646

UniProt: Q96RU2