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Layilin anticorps (AA 281-382)

Name: Layilin anticorps (AA 281-382)
SKU: ABIN707756
Price: 414.62 EUR
Availability: InStock

LAYN Reactivité: Souris WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) Hôte: Lapin Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with (Layilin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin for 40 minutes and DAPI staining of the nuclei. (Layilin anticorps (AA 281-382))

IF (p)

Paraformaldehyde-fixed, paraffin embedded Mouse small intestine tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with Layilin Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (Layilin anticorps (AA 281-382))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse intestines, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with (Layilin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin for 40 minutes and DAPI staining of the nuclei. (Layilin anticorps (AA 281-382))

IF (p)

3 images

N° du produit ABIN707756

Fiche de données

Antigène Voir toutes Layilin (LAYN) Anticorps

Layilin (LAYN)
Épitope
4

3

2

2

1

1

1

1
AA 281-382
Reactivité
21

10

4

2

1

1

1

1

1
Souris
Hôte
16

5
Lapin
Anticorps secondaires appropriés
Clonalité
18

3
Polyclonal
Conjugué
11

2

1

1

1

1

1

1

1

1
Cet anticorp Layilin est non-conjugé

Application

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Réactivité croisée

Souris

Homologie

Human,Rat,Dog,Cow,Horse,Chicken,Rabbit

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from human Layilin

Isotype

IgG

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anti-Layilin (LAYN) (Internal Region) antibody
LAYN Reactivité: Humain, Souris, Rat WB, ELISA, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Layilin (LAYN) (AA 1-374) antibody
LAYN Reactivité: Humain WB Hôte: Souris Polyclonal unconjugated

anti-Layilin (LAYN) (Internal Region) antibody
LAYN Reactivité: Humain WB, ELISA Hôte: Lapin Polyclonal unconjugated

anti-Layilin (LAYN) (AA 262-382) antibody
LAYN Reactivité: Humain ELISA, IF Hôte: Lapin Polyclonal unconjugated

anti-Layilin (LAYN) (AA 41-90) antibody
LAYN Reactivité: Humain, Souris, Rat, Cobaye, Porc WB Hôte: Lapin Polyclonal unconjugated

anti-Layilin (LAYN) (AA 71-120), (Internal Region) antibody
LAYN Reactivité: Humain, Souris, Rat WB, ELISA, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Layilin (LAYN) (AA 262-382) antibody (HRP)
LAYN Reactivité: Humain ELISA Hôte: Lapin Polyclonal HRP

anti-Layilin (LAYN) (AA 262-382) antibody (FITC)
LAYN Reactivité: Humain Hôte: Lapin Polyclonal FITC

anti-Layilin (LAYN) (AA 262-382) antibody (Biotin)
LAYN Reactivité: Humain ELISA Hôte: Lapin Polyclonal Biotin

anti-Layilin (LAYN) (AA 1-374) antibody
LAYN Reactivité: Humain WB Hôte: Souris Polyclonal unconjugated

Indications d'application

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Antigène

Layilin (LAYN)

Autre désignation

Layilin (LAYN Produits)

Synonymes

anticorps LAYN, anticorps si:dkey-27p23.2, anticorps E030012M19Rik, anticorps Gm511, anticorps RGD1564444, anticorps layilin, anticorps layilin a, anticorps LAYN, anticorps layna, anticorps Layn

Sujet

Synonyms: Layn, E030012M19Rik, Gm511.
Background: LAYN is integral membrane protein, and a hyaluronate receptor. It has been shown to bind specifically to hyaluronan but not to other glycosaminoglycans. It is a recently characterized transmembrane protein with homology to C type lectins, and is a membrane binding site for talin in peripheral ruffles of spreading cells. LAYN may have a role in cell adhesion and motility.

ID gène

143903