MICA anticorps (AA 1-200)

N° du produit ABIN7504179

Détail du produit anti-MICA anticorps

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Épitope: AA 1-200

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MICA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: MICA and MICB are stress-induced antigens that are related to major histocompatibility complex (MHC) class I molecules. MICA and MICB are frequently expressed in epithelial tumors. These highly glycosylated cell surface proteins are stably expressed without conventional class I peptide ligands or association with i²-2-microglobulin. The expression is induced on proliferating or heat shock-stressed epithelial cells. MICA and MICB are broadly recognized by intestinal epithelial V L1 i³ L T cells expressing variable TCRs, suggesting that these antigens may play a central role in the signaling of cellular distress to evoke immune responses in the intestinal epithelium.

Réactivité croisée (Details): Human, Mouse and Rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa1-200) of human MICA (exact sequence is proprietary)

Isotype: IgG2b

Information d'application

Indications d'application:

Positive Control: HeLa or MCF-7 cells. Human breast, kidney or prostate.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur MICA

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Autre désignation: MICA (MICA Produits)

Synonymes: anticorps MIC-A, anticorps PERB11.1, anticorps MHC class I polypeptide-related sequence A, anticorps MICA

Sujet: HLA class I antigen, MHC class I chain related gene A protein antibody, MHC class I chain related protein A, MHC class I chain related protein A HLA B HLA C, MHC class I polypeptide related sequence A, MHC class I polypeptide-related sequence A, MHC class I related protein, MIC A, MIC-A, micA, PERB11.1, Stress inducible class I homolog,MICA
Cellular localisation: Cell surface. Cytoplasm.

Poids moléculaire: 92kDa

ID gène: 100507436

UniProt: Q29983

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis