CFTR anticorps (AA 258-385)

N° du produit ABIN7504154

L’anticorps anti-CFTR Monoclonal Souris est utilisé pour la détection de CFTR dans des échantillons de Humain. Il a été validé pour IHC (f).

Aperçu rapide pour CFTR anticorps (AA 258-385) (ABIN7504154)

Antigène: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CFTR est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-CFTR anticorps

Épitope: AA 258-385

Specificité: Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified by Protein A.

Immunogène: Recombinant fragment (around aa 258-385) of human CFTR protein (exact sequence is proprietary)

Isotype: IgG1

Information d'application

Indications d'application:

Positive Control: MOLT-4 cells. Human pancreas, kidney or placenta.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur CFTR

Antigène: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Autre désignation: CFTR

Sujet: ABC35, ATP Binding Cassette Superfamily C Member 7 (ABCC7), cAMP-dependent chloride channel, CFTR, CFTR/MRP, Channel conductance-controlling ATPase, Cystic Fibrosis Transmembrane Conductance Regulator, MRP7, TNR CFTR,CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)
Cellular localisation: Cell Surface. Cytoplasm.

Poids moléculaire: 165-170kDa

ID gène: 1080, 489786, 621460

UniProt: P13569