UBE2C anticorps

N° du produit ABIN7504126

Détail du produit anti-UBE2C anticorps

Antigène: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp UBE2C est non-conjugé

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Specificité: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ubconjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.

Réactivité croisée (Details): Human

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human full-length UBE2C protein

Isotype: IgG2b

Information d'application

Indications d'application:

Positive Control: HeLa, Jurkat or A431 cells. Human tonsil or placenta tissue.

Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur UBE2C

Antigène: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Autre désignation: UBE2C (UBE2C Produits)

Synonymes: anticorps LOC458287, anticorps UBCH10, anticorps dJ447F3.2, anticorps 1110015A16Rik, anticorps D2Ertd695e, anticorps im:7137135, anticorps zgc:123190, anticorps ubiquitin conjugating enzyme E2 C, anticorps ubiquitin-conjugating enzyme E2C, anticorps ubiquitin conjugating enzyme E2 C L homeolog, anticorps ubiquitin conjugating enzyme E2 C S homeolog, anticorps UBE2C, anticorps NAEGRDRAFT_82946, anticorps ube2c.L, anticorps Ube2c, anticorps ube2c.S, anticorps ube2c

Sujet: Cyclin selective ubiquitin carrier protein, mitotic specific ubiquitin conjugating enzyme, UB E2C, UBCH10, Ubiquitin protein ligase C,Ubiquitin-conjugating Enzyme E2C / UBCH10
Cellular localisation: Cytoplasm.

Poids moléculaire: 20kDa

ID gène: 11065, 93002

UniProt: O00762

Pathways: Ubiquitin Proteasome Pathway