TdT anticorps (AA 52-192)

N° du produit ABIN7126302

L’anticorps Souris Monoclonal anti-TdT a été validé pour ELISA, IHC (f) et Coat. Il convient pour détecter TdT dans des échantillons de Humain.

Aperçu rapide pour TdT anticorps (AA 52-192) (ABIN7126302)

Antigène: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TdT est non-conjugé

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Détail du produit anti-TdT anticorps

Épitope: AA 52-192

Specificité: Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL).

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa 52-192) of human DNTT protein (exact sequence is proprietary)

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human thymus. Jurkat cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur TdT

Antigène: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Autre désignation: DNTT

Sujet: Deoxynucleotidyltransferase terminal, DNTT, Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase, TDT, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal deoxyribonucleotidyltransferase,TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker)
Cellular localisation: Nucleus.

Poids moléculaire: 58kDa

ID gène: 1791, 534206

UniProt: P04053

Pathways: Réparation de l'ADN