Recombinant AMACR anticorps

N° du produit ABIN7126435

Cet anticorps anti-AMACR est un anticorps Souris Monoclonal détectant AMACR dans WB et IHC (f). Adapté pour Humain.

Aperçu rapide pour Recombinant AMACR anticorps (ABIN7126435)

Antigène: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AMACR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-AMACR anticorps

Specificité: This antibody recognizes a protein of 42 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and i²-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human AMACR protein

Isotype: IgG2a

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: PC3 or HEK cells. Human kidney, liver or prostate adenocarcinoma. Human kidney lysate.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur AMACR

Antigène: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Autre désignation: AMACR

Sujet: Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM,AMACR / p504S (Prostate Cancer Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 42kDa

ID gène: 23600, 508343

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process