CNOT10 anticorps

N° du produit ABIN7126458

L’anticorps anti-CNOT10 Monoclonal Souris est utilisé pour la détection de CNOT10 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IF et FACS.

Aperçu rapide pour CNOT10 anticorps (ABIN7126458)

Antigène: CNOT10 (CCR4-NOT Transcription Complex, Subunit 10 (CNOT10))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CNOT10 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-CNOT10-1D5

Détail du produit anti-CNOT10 anticorps

Fonction: CCR4-NOT transcription complex, subunit 10 (CNOT10) Antibody

Specificité: CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human CNOT10 protein

Isotype: IgG2c

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CNOT10

Antigène: CNOT10 (CCR4-NOT Transcription Complex, Subunit 10 (CNOT10))

Autre désignation: CNOT10

Sujet:

FLJ12890, FLJ13165

HeLa or HepG2 cells.

Poids moléculaire: 82.31kDa

ID gène: 25904, 444851

UniProt: Q9H9A5