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GTF2B anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement GTF2B dans ELISA, IP, FACS et Coat. Il présente une réactivité envers Humain.
N° du produit ABIN7126496

Aperçu rapide pour GTF2B anticorps (ABIN7126496)

Antigène

Voir toutes GTF2B Anticorps
GTF2B (General Transcription Factor IIB (GTF2B))

Reactivité

  • 78
  • 31
  • 16
  • 13
  • 9
  • 8
  • 8
  • 8
  • 7
  • 6
  • 6
  • 5
  • 4
  • 4
  • 1
Humain

Hôte

  • 64
  • 14
Souris

Clonalité

  • 64
  • 14
Monoclonal

Conjugué

  • 44
  • 5
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp GTF2B est non-conjugé

Application

  • 59
  • 24
  • 13
  • 13
  • 13
  • 12
  • 7
  • 7
  • 5
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)
  • Specificité

    In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.

    Réactivité croisée (Details)

    Human.

    Purification

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogène

    Recombinant full-length human GTF2Bprotein

    Isotype

    IgG2b
  • Indications d'application

    Known_Application: ELISA (For coating, order antibody without BSA), ,Flow Cytometry (1-2 μg/million cells), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa or MCF-7 cells.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Agent conservateur

    Azide free

    Stock

    -20 °C,-80 °C

    Stockage commentaire

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Date de péremption

    24 months
  • Antigène

    GTF2B (General Transcription Factor IIB (GTF2B))

    Autre désignation

    GTF2B

    Sujet

    General transcription factor IIB, General transcription factor TFIIB, gtf2b, RNA polymerase II transcription factor IIB, S300 II, S300-II, TF IIB, TF2B, TFIIB, Transcription initiation factor IIB,GTF2B/ TFIIB (Transcription Factor 2B)
    Cellular localisation: Nucleus. Nucleoplasm, nuclear bodies.

    Poids moléculaire

    34.8kDa

    ID gène

    2959, 481852

    UniProt

    Q00403
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