APP anticorps (AA 578-680)

N° du produit ABIN7126553

Cet anticorps Souris Monoclonal détecte spécifiquement APP dans IF, FACS et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour APP anticorps (AA 578-680) (ABIN7126553)

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APP est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-APP anticorps

Épitope: AA 578-680

Specificité: Proteolytic cleavage of the Amyloid protein precursor (APP) gives rise to the i²-Amyloid and Amyloid A4 proteins, which are present in human platelets. Amyloid deposition is associated with type II diabetes, Down syndrome and a variety of neurological disorders, including Alzheimer s disease. The Amyloid precursor protein (APP) undergoes alternative splicing, resulting in several isoforms. Proteolytic cleavage of APP leads to the formation of the 4 kDa Amyloid i²/A4 Amyloid protein. This protein is involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer s patients. APLP1 (Amyloid precursor-like protein 1) and APLP2 are structurally similar to APP. Human APLP2 is a membrane-bound sperm protein that contains a region highly homologous to the transmembrane-cytoplasmic domains of APP found in brain plaques of Alzheimer s disease patients.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa578-680) of human Amyloid Beta protein (exact sequence is proprietary)

Isotype: IgG2

Information d'application

Indications d'application:

Known_Application: Immunofluorescence (1-2ug/ml); Flow Cytometry (1-2ug/million cells);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Alzheimer's disease brain. Amyloidosis.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur APP

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Autre désignation: APP

Sujet: CVAP, AAA, AICD-50, PN2, 50, Beta-APP42, AID, S-APP-alpha, 57, AD1, PN-II, Beta-APP40, 42, 40, APPI, Alzheimer disease amyloid protein, PreA4, ABETA, Amyloid intracellular domain 50, 57, 59 AICD-59, S-APP-beta, APP, AICD-57, ABPP, Protease nexin-II, Cerebral vascular amyloid peptide,beta Amyloid
Cellular localisation: Membrane. Membrane > clathrin-coated pit. Cell surface protein that rapidly becomes internalized via clathrin-coated pits.

Poids moléculaire: 4-46kDa (i²-Amyloid), 100-125kDa (Amyloid A4)

ID gène: 351, 434980

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour