Recombinant Prostate Specific Antigen anticorps (AA 150-250)

N° du produit ABIN7126557

L’anticorps Lapin Monoclonal anti-Prostate Specific Antigen a été validé pour IHC (f). Il convient pour détecter Prostate Specific Antigen dans des échantillons de Humain.

Aperçu rapide pour Recombinant Prostate Specific Antigen anticorps (AA 150-250) (ABIN7126557)

Antigène: Prostate Specific Antigen (PSA)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Prostate Specific Antigen est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-Prostate Specific Antigen anticorps

Épitope: AA 150-250

Specificité: Prostate-specific antigen (PSA) is a single-chain glycoprotein of 237 amino acids containing approximately 8 % carbohydrate. It is a serine protease produced almost exclusively by prostatic epithelial cells. Immunohistochemically PSA is expressed in the highly specialized apically-superficial layer of female and male secretory cells of the prostate gland, and is readily demonstrated in adenocarcinomas of the prostate in about 99 % of the cases. There is a correlation between malignancy grade and intensity of staining, high grade carcinomas displaying weaker expression. About 1 % of poorly differentiated carcinomas have been negative for PSA. Due to the high specificity of PSA for prostatic glandular epithelium, it is very useful in identifying prostatic carcinoma in the prostate and in the adjacent organs often affected by epithelial malignancies, i.e. rectum and urinary bladder. PSA may be used in a panel together with NKX3.1 and Prostein, which are at least as sensitive and slightly more specific than PSA.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Synthetic peptide corresponding to PSA residues within aa150-250 of PSA was used as an immunogen.

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: PC12 cells. Normal prostate or prostate carcinoma tissues.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Prostate Specific Antigen

Antigène: Prostate Specific Antigen (PSA)

Autre désignation: KLK3

Sujet: Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin,Prostate Specific Antigen (PSA)
Cellular localisation: Secreted

Poids moléculaire: 29kDa

ID gène: 354, 171995

UniProt: P07288

Pathways: Système du Complément