Recombinant Cytokeratin 5 anticorps (AA 316-590)

N° du produit ABIN7126613

Cet anticorps Souris Monoclonal détecte spécifiquement Cytokeratin 5 dans IHC et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Cytokeratin 5 anticorps (AA 316-590) (ABIN7126613)

Antigène: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 5 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-Cytokeratin 5 anticorps

Épitope: AA 316-590

Specificité: This Mab recognizes a protein of 58 kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, yoepithelial, and mesothelium.Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)

Isotype: IgG2a

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, esophagus or bladder tissue (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Cytokeratin 5

Antigène: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Autre désignation: KRT5

Sujet: 58 kDa Cytokeratin, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, Type-II Cytoskeletal 5, Type-II keratin Kb5,Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 58kDa

ID gène: 3852, 433845

UniProt: P13647