MBP anticorps

N° du produit ABIN7126669

Cet anticorps anti-MBP Monoclonal Souris (Clone MBP-4271) (ABIN7126669) détecte spécifiquement MBP dans WB, IHC et ELISA. L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Boeuf (Vache) et Hamster.

Aperçu rapide pour MBP anticorps (ABIN7126669)

Antigène: MBP

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MBP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: MBP-4271

Détail du produit anti-MBP anticorps

Fonction: Myelin Basic Protein Antibody

Specificité: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4271 recognizes an epitope in the 129-138 region of MBP, useful in clinical diagnosis to detect MBP levels.

Réactivité croisée (Details): Human, bovine and rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Reacts with MBP from human, bovine and rat, recognizing epitope 129-138

Isotype: IgG2a

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Immunocytochemistry (0.5-1 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human brain or astrocytoma tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MBP

Antigène: MBP

Sujet:

GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP

Human brain or astrocytoma tissue. Mouse Brain, Rat Brain or Hamster Brain

Poids moléculaire: 14-22kDa

ID gène: 4155, 551713

UniProt: P02686