MBP anticorps (AA 119-131)

N° du produit ABIN7126670

Cet anticorps Souris Monoclonal détecte spécifiquement MBP dans WB, IHC, ELISA et IF. Il présente une réactivité avec des échantillons de Humain, Rat, Souris, Cobaye, Boeuf (Vache), Hamster et Lapin.

Aperçu rapide pour MBP anticorps (AA 119-131) (ABIN7126670)

Antigène: MBP

Reactivité: Humain, Rat, Souris, Cobaye, Boeuf (Vache), Hamster, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MBP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: MBP-4272

Détail du produit anti-MBP anticorps

Épitope: AA 119-131

Fonction: Myelin Basic Protein Antibody

Specificité: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4272 recognizes an epitope in the 119-131 region of MBP, useful in clinical diagnosis to detect MBP levels in human, rat and cow MBP.

Réactivité croisée (Details): Human, bovine and rat, reacts weakly with rabbit. Does not react with guinea pig.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recognizes bovine BMP, in the region of residues 119-131 (GAEGQRPGFGYGG).

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U87 cells. Human brain or astrocytoma tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MBP

Antigène: MBP

Sujet:

GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP

U87 cells. Human brain or astrocytoma tissue. Mouse Brain, Rat Brain, Guinea pig Brain or Hamster Brain

Poids moléculaire: 14-22kDa

ID gène: 4155, 551713

UniProt: P02686