Recombinant Geminin anticorps (AA 71-202)

N° du produit ABIN7126767

L’anticorps Lapin Monoclonal anti-Geminin a été validé pour IHC (f). Il convient pour détecter Geminin dans des échantillons de Humain.

Aperçu rapide pour Recombinant Geminin anticorps (AA 71-202) (ABIN7126767)

Antigène: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Geminin est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-Geminin anticorps

Épitope: AA 71-202

Specificité: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa71-202) of human GMNN protein (exact sequence is proprietary)

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293, HeLa or MCF-7 cells. Human tonsil, testis or triple-negative breast cancer tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Geminin

Antigène: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Autre désignation: GMNN

Sujet: DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GMNN, RP3 369A17.3,Geminin / DNA Replication Inhibitor
Cellular localisation: Nuclear expression in highly proliferating cells.

Poids moléculaire: 35kDa

ID gène: 51053, 234896

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication