Recombinant CD31 anticorps (AA 28-330)

N° du produit ABIN7126782

L’anticorps Souris Monoclonal anti-CD31 a été validé pour FACS, WB et IHC (f). Il convient pour détecter CD31 dans des échantillons de Humain.

Aperçu rapide pour Recombinant CD31 anticorps (AA 28-330) (ABIN7126782)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-CD31 anticorps

Épitope: AA 28-330

Specificité: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Réactivité croisée (Details): Human. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (aa 28-330) of human CD31 expressed in E. Coli (exact sequence is proprietary)

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Western Blotting (1-2 μg/mL),Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: PECAM1

Sujet: EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA',CD31 / PECAM-1 (Endothelial Cell Marker)
Cellular localisation: Cell surface and cytoplasm of endothelial cells

Poids moléculaire: ~100kDa (endothelium) and ~130kDa (platelets)

ID gène: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization