POLE3 anticorps

N° du produit ABIN7126801

Cet anticorps anti-POLE3 est un anticorps Souris Monoclonal détectant POLE3 dans IF, FACS et IHC (f). Adapté pour Humain.

Aperçu rapide pour POLE3 anticorps (ABIN7126801)

Antigène: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POLE3 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-POLE3 anticorps

Specificité: DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.

Réactivité croisée (Details): Human. Predicted to react in Mouse, Rat and Xenopus.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human POLE3 protein

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur POLE3

Antigène: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Autre désignation: POLE3

Sujet: Arsenic transactivated protein, ASTP, CHRAC17, Chromatin accessibility complex 17 kDa protein, DNA polymerase II subunit 3, Histone fold protein CHRAC17, HuCHRAC17, Polymerase (DNA directed) epsilon 3 (POLE3), YBL1,POLE3 / CHRAC17
Cellular localisation: Nucleus. Cytoplasm.

Poids moléculaire: 16.86kDa

ID gène: 54107, 108112

UniProt: Q9NRF9

Pathways: Réparation de l'ADN