POLE3 anticorps

N° du produit ABIN7126802

Cet anticorps Souris Monoclonal détecte spécifiquement POLE3 dans IF, ELISA et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour POLE3 anticorps (ABIN7126802)

Antigène: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POLE3 est non-conjugé

Application: Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Clone: PCRP-POLE3-3D3

Détail du produit anti-POLE3 anticorps

Fonction: POLE3 / CHRAC17 Antibody

Specificité: DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.

Réactivité croisée (Details): Human. Predicted to react in Mouse, Rat and Xenopus.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human POLE3 protein

Isotype: IgG2a

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur POLE3

Antigène: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Autre désignation: POLE3

Sujet:

Arsenic transactivated protein, ASTP, CHRAC17, Chromatin accessibility complex 17 kDa protein, DNA polymerase II subunit 3, Histone fold protein CHRAC17, HuCHRAC17, Polymerase (DNA directed) epsilon 3 (POLE3), YBL1

HeLa or K562 cells.

Poids moléculaire: 16.86kDa

ID gène: 54107, 108112

UniProt: Q9NRF9

Pathways: Réparation de l'ADN