Recombinant Perforin 1 anticorps (AA 413-552)

N° du produit ABIN7126823

Cet anticorps Lapin Monoclonal détecte spécifiquement Perforin 1 dans IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Perforin 1 anticorps (AA 413-552) (ABIN7126823)

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Perforin 1 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-Perforin 1 anticorps

Épitope: AA 413-552

Specificité: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human Perforin-1protein fragment (around aa 413-552) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HDLM-2cells. Human spleen.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Perforin 1

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Autre désignation: PRF1

Sujet: Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein, PRF1 (pore forming protein 1), Perforin-1, PFP, PGFL, PIGF, PIGF-2, PLGF,Perforin-1 (Pore Forming Protein) (Apoptosis Marker)
Cellular localisation: Cytoplasm.

Poids moléculaire: 75kDa

ID gène: 5551, 2200

UniProt: P14222

Pathways: Apoptose, Caspase Cascade in Apoptosis