Actin anticorps

N° du produit ABIN7126863

L’anticorps anti-Actin Monoclonal Souris est utilisé pour la détection de Actin dans des échantillons de Humain, Souris, Rat, Cobaye et Hamster. Il a été validé pour WB, IHC, IF et FACS.

Aperçu rapide pour Actin anticorps (ABIN7126863)

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivité: Humain, Souris, Rat, Cobaye, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ACTA1-360

Détail du produit anti-Actin anticorps

Fonction: Actin alpha 1, Skeletal Muscle Antibody

Specificité: All eukaryotic cells express Actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Réactivité croisée (Details): Human.Predicted to show a broad reactivity.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human ACTA1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Skeletal muscle.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Actin

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Autre désignation: ACTA1

Sujet:

a actin, ACTA, ACTA1, Actin, alpha skeletal muscle, ACTS_HUMAN, Alpha actin 1, Alpha-actin-1, ASMA, MPFD, Skeletal muscle alpha actin 1

Hela or T47D cells, Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine, Human skeletal muscle, Mouse skeletal muscle, Rat skeletal muscle, Hamster skeletal muscle or Guinea pig skeletal muscle

Poids moléculaire: 43kDa

ID gène: 58, 1288

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development