beta Actin anticorps

N° du produit ABIN7126900

Cet anticorps anti-beta Actin est un anticorps Souris Monoclonal détectant beta Actin dans WB, IF, FACS et IHC (f). Adapté pour Humain.

Aperçu rapide pour beta Actin anticorps (ABIN7126900)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-beta Actin anticorps

Specificité: All eukaryotic cells express Actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas i²-Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Réactivité croisée (Details): Human, Mouse and Rat. Predicted to react in Xenopus, Bovine, Chicken, Horse, Monkey, Pig and Sheep.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human ACTB protein

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human myoepithelial cells. HeLa, Jurkat or Raji cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: ACTB

Sujet: A26C1A, A26C1B, ACTB, Actin beta, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, Melanoma X actin, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1,Beta-Actin (ACTB)
Cellular localisation: Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Poids moléculaire: 43kDa

ID gène: 60, 708120

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction