Osteopontin anticorps

N° du produit ABIN7126977

Cet anticorps Rat Monoclonal détecte spécifiquement Osteopontin dans WB, FACS, IF, Func et IHC (f). Il présente une réactivité envers Rat.

Aperçu rapide pour Osteopontin anticorps (ABIN7126977)

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivité: Rat

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Osteopontin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: OSP-4589

Détail du produit anti-Osteopontin anticorps

Specificité: Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

Réactivité croisée (Details): Human, Dog, Mink, Mouse, Rabbit, Rat and Turkey.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length rat Spp1 protein

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Functional Studies, Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Bone. Found in plasma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Osteopontin

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Autre désignation: SPP1

Sujet: BNSP, Bone sialoprotein 1 (BSPI/BSP1), Early T lymphocyte activation 1 (ETA1), Nephropontin, immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (SPP1), CALPHA1 fusion, Urinary stone protein, Uropontin,Osteopontin (OSP) / Secreted Phosphoprotein 1 (SPP1)
Cellular localisation: Secreted.

Poids moléculaire: 34.9kDa

ID gène: 6696, 313

UniProt: P10451

Pathways: Regulation of Cell Size