GTF2H2C anticorps

N° du produit ABIN7127037

Détail du produit anti-GTF2H2C anticorps

Antigène: GTF2H2C (General Transcription Factor IIH, Polypeptide 2C (GTF2H2C))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GTF2H2C est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Réactivité croisée (Details): Human. Predicted to react in Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human GTF2H2C protein

Isotype: IgG2

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Raji cells. Human breast or prostate carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur GTF2H2C

Antigène: GTF2H2C (General Transcription Factor IIH, Polypeptide 2C (GTF2H2C))

Autre désignation: GTF2H2C (GTF2H2C Produits)

Synonymes: anticorps GTF2H2 family member C, anticorps GTF2H2C

Sujet: EVR6, PFM14, PR domain zinc finger protein 17 (PRDM17), RP72, Zinc finger protein 408 (GTF2H2C),GTF2H2C (Transcription Factor)
Cellular localisation: Nucleus. Nuclear speckles.

Poids moléculaire: 44kDa

ID gène: 730394, 422901

UniProt: Q6P1K8