ube3a anticorps

N° du produit ABIN7127039

Cet anticorps anti-ube3a est un anticorps Souris Monoclonal détectant ube3a dans IF et FACS. Adapté pour Humain.

Aperçu rapide pour ube3a anticorps (ABIN7127039)

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ube3a est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-ube3a anticorps

Specificité: E6-associating protein (E6-AP), also designated ubiquitin protein ligase E3A(UBE3A), is a component of the ubiquitin-mediated proteolytic pathway thatselectively targets proteins for degradation by the 26S Proteasome. Ubiquitin(Ub) is directly conjugated to protein substrates by the transfer of Ub from anE2 ubiquitin conjugating enzyme to the target protein. This conjugation is facil-itated by the enzymatic activity of E3 ubiquitin ligase family members such asE6-AP. Several substrates of E6-AP have been identified and include the tumorsuppressor protein p53 and the mammalian homolog of Rad23, HHR23A.Previous studies have indicated that E6-AP associates with the humanpapillomavirus E6 oncogene, which forms a complex with p53 and therebypotentiates E6-AP mediated ubiquitination of p53. Genetic mutations thatimpair E6-AP activity result in the accumulation of p53 in the cytoplasm, andin many instances, these mutations are associated with the development ofthe rare neurodevelopmental disorder Angelman syndrome (AS), which ischaracterized by severe motor dysfunction and mental retardation.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human UBE3A protein

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur ube3a

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Autre désignation: UBE3A

Sujet: ANCR, Angelman syndrome (AS), CTCL tumor antigen se37-2, E6AP ubiquitin protein ligase, EPVE6AP, FLJ26981, HECT-type ubiquitin transferase E3A, Human papilloma virus E6 associated protein Angelman syndrome, HPVE6A, NY REN 54 antigen, Oncogenic protein associated protein E6AP, Renal carcinoma antigen NY REN 54,UBE3A / E6-AP
Cellular localisation: Nucleus. Cytoplasm.

Poids moléculaire: 100kDa

ID gène: 7337, 598862

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway