CA9 anticorps (AA 314-410)
Aperçu rapide pour CA9 anticorps (AA 314-410) (ABIN7127067)
Antigène
Voir toutes CA9 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 314-410
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Fonction
- Renal Cell Carcinoma (Carbonic Anhydrase IX) Antibody
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Specificité
- Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200).In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
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Réactivité croisée (Details)
- Human.
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Purification
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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Immunogène
- Recombinant human CA9 protein fragment (around aa314-410) (exact sequence is proprietary)
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Isotype
- IgG2b, kappa
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Indications d'application
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Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Normal kidney or renal cell carcinoma (IHC).
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Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS
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Agent conservateur
- Azide free
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Date de péremption
- 24 months
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- CA9 (Carbonic Anhydrase IX (CA9))
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Autre désignation
- CA9
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Sujet
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CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, G250, Membrane antigen MN,P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250
Normal kidney or renal cell carcinoma (IHC).
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Poids moléculaire
- 55kDa
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ID gène
- 768, 63287
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UniProt
- Q16790
Antigène
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