Recombinant Caldesmon anticorps

N° du produit ABIN7127077

L’anticorps Souris Monoclonal anti-Caldesmon a été validé pour WB et IHC (f). Il convient pour détecter Caldesmon dans des échantillons de Humain.

Aperçu rapide pour Recombinant Caldesmon anticorps (ABIN7127077)

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caldesmon est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-Caldesmon anticorps

Specificité: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Réactivité croisée (Details): Human. Does not react with rat. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human CALD1 protein

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistology (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ovary, Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Caldesmon

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Autre désignation: CALD1

Sujet: CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22,Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 120-150kDa

ID gène: 800, 490203

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction