Recombinant CD8 alpha anticorps (AA 135-235)

N° du produit ABIN7127153

Cet anticorps anti-CD8 alpha est un anticorps Lapin Monoclonal détectant CD8 alpha dans IHC (f). Adapté pour Humain.

Aperçu rapide pour Recombinant CD8 alpha anticorps (AA 135-235) (ABIN7127153)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-CD8 alpha anticorps

Épitope: AA 135-235

Specificité: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that serves as a coreceptor for TCR recognition of MHC class I associated peptides and supports CTL activation by binding to the MHC, while making no direct contact with the peptide.CD8 is expressed on cytotoxic suppressor T cells. It is expressed as a heterodimer of CD8i± and CD8i². CD8 is expressed on approximately one-third of peripheral blood T cells (the CD4-negative cells). CD8 has also been detected at a low level on some natural killer (NK) cells. In normal human tonsil, large numbers of CD8+ lymphocytes were present within the paracortex, occasionally positive cells were also identified within germinal centers and within the investing squamous epithelium. In other tissues, only lymphoid cells and cells of histiocyte lineage showed positive staining for CD8.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Synthetic peptide corresponding to CD8α residues within aa135-235 of CD8α

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8A

Sujet: CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain,CD8A (Cytotoxic / Suppressor T-Cell Marker)
Cellular localisation: Cell surface

Poids moléculaire: 32kDa

ID gène: 925, 85258

UniProt: P01732

Pathways: TCR Signaling