MED7 anticorps

N° du produit ABIN7127191

L’anticorps Souris Monoclonal anti-MED7 a été validé pour ELISA, FACS, IF et IP. Il convient pour détecter MED7 dans des échantillons de Humain.

Aperçu rapide pour MED7 anticorps (ABIN7127191)

Antigène: MED7 (Mediator Complex Subunit 7 (MED7))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MED7 est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-MED7 anticorps

Specificité: MED7, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, MED7 functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, MED7 regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. MED7 may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases MED7 mRNA-binding affinity. Defects or deletions in the gene encoding MED7 are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of MED7 exist due to alternative splicing events.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human MED7 protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: ELISA (use assay dependent concentration), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U-251 MG or CACO-2 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MED7

Antigène: MED7 (Mediator Complex Subunit 7 (MED7))

Autre désignation: MED7

Sujet: Activator recruited cofactor 34 kDa component, ARC34, Cofactor required for Sp1 transcriptional activation, subunit 9, 33 kDa, CRSP complex subunit 9, CRSP9, hMED7, Mediator of RNA polymerase II transcription subunit 7, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33,MED7
Cellular localisation: Nucleus. Nuclear bodies.

Poids moléculaire: 27.2kDa

ID gène: 9443, 279902

UniProt: O43513

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha