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Recombinant Keratin 77 anticorps (AA 100-200)

Cet anticorps anti-Keratin 77 est un anticorps Lapin Monoclonal détectant Keratin 77 dans IHC (f). Adapté pour Humain.
N° du produit ABIN7127261

Aperçu rapide pour Recombinant Keratin 77 anticorps (AA 100-200) (ABIN7127261)

Antigène

Voir toutes Keratin 77 (KRT77) Anticorps
Keratin 77 (KRT77)

Type d'anticorp

Recombinant Antibody

Reactivité

  • 19
  • 7
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Humain

Hôte

  • 11
  • 8
Lapin

Clonalité

  • 10
  • 9
Monoclonal

Conjugué

  • 15
  • 2
  • 1
  • 1
Cet anticorp Keratin 77 est non-conjugé

Application

  • 11
  • 10
  • 7
  • 6
  • 5
  • 3
  • 2
  • 1
Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
  • Épitope

    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 100-200

    Specificité

    The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.

    Réactivité croisée (Details)

    Human.

    Purification

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogène

    Synthetic peptide corresponding to Type I Cytokeratins residues within aa100-200 of Type I Cytokeratins

    Isotype

    IgG
  • Indications d'application

    Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Positive_Control: Skin or squamous cell carcinoma.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Agent conservateur

    Azide free

    Stock

    -20 °C,-80 °C

    Stockage commentaire

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Date de péremption

    24 months
  • Antigène

    Keratin 77 (KRT77)

    Autre désignation

    KRT77

    Sujet

    K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B,Cytokeratin, Type I (Epithelial Marker)
    Cellular localisation: Cytoplasmic

    Poids moléculaire

    60/53/51/45/40kDa

    ID gène

    374454, 334989

    UniProt

    Q7Z794
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