Recombinant Retinoblastoma 1 anticorps (pSer780)

N° du produit ABIN7127740

Cet anticorps anti-Retinoblastoma 1 est un anticorps Lapin Monoclonal détectant Retinoblastoma 1 dans IHC, ELISA, IF et IP. Adapté pour Humain.

Aperçu rapide pour Recombinant Retinoblastoma 1 anticorps (pSer780) (ABIN7127740)

Antigène: Retinoblastoma 1 (RB1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Retinoblastoma 1 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 2E9

Détail du produit anti-Retinoblastoma 1 anticorps

Épitope: pSer780

Fonction: Phospho-RB1 (S780) Recombinant Monoclonal Antibody

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Phospho-RB1 (S780)

Isotype: IgG

Information d'application

Indications d'application: IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur Retinoblastoma 1

Antigène: Retinoblastoma 1 (RB1)

Autre désignation: RB1

Sujet:

Background: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Aliases: Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1

UniProt: P06400

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy