Recombinant Topoisomerase I anticorps

N° du produit ABIN7127850

L’anticorps Lapin Monoclonal anti-Topoisomerase I a été validé pour WB, ELISA, IHC, IP et FACS. Il convient pour détecter Topoisomerase I dans des échantillons de Humain.

Aperçu rapide pour Recombinant Topoisomerase I anticorps (ABIN7127850)

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase I est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 6D8

Détail du produit anti-Topoisomerase I anticorps

Fonction: TOP1 Recombinant Monoclonal Antibody

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human TOP1

Isotype: IgG

Information d'application

Indications d'application: WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200, IP:1:200-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur Topoisomerase I

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Autre désignation: TOP1

Sujet:

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Aliases: DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I), TOP1

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance