His Tag anticorps
Aperçu rapide pour His Tag anticorps (ABIN7131650)
Antigène
Voir toutes His Tag AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Purification
- Protein A purified
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Immunogène
- HHHHHH(6x His) synthetic peptide conjugate to KLH
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Isotype
- IgG2a
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Indications d'application
- Recommended dilution: WB:1:500-1:2000,
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4 -
Agent conservateur
- ProClin
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Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- His Tag
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Autre désignation
- 6*His
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Classe de substances
- Tag
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Sujet
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Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag
Antigène
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