TOP1MT anticorps (AA 270-400) (Biotin)

N° du produit ABIN7150352

Cet anticorps Lapin Polyclonal détecte spécifiquement TOP1MT dans ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour TOP1MT anticorps (AA 270-400) (Biotin) (ABIN7150352)

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP1MT est conjugé à/à la Biotin

Application: ELISA

Détail du produit anti-TOP1MT anticorps (Biotin)

Épitope: AA 270-400

Fonction: TOP1MT Antibody, Biotin conjugated

Purification: Protein G purified

Pureté: >95 %

Immunogène: Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur TOP1MT

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Autre désignation: TOP1MT

Sujet:

Background: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3\'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5\'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5\'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Aliases: TOP1MT antibody, DNA topoisomerase I antibody, mitochondrial antibody, TOP1mt antibody, EC 5.6.2.1 antibody

UniProt: Q969P6