PKM anticorps

N° du produit ABIN7163482

Cet anticorps anti-PKM est un anticorps Souris Monoclonal détectant PKM dans ELISA, WB et IP. Adapté pour Humain.

Aperçu rapide pour PKM anticorps (ABIN7163482)

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PKM est non-conjugé

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-PKM anticorps

Fonction: PKM Monoclonal Antibody

Purification: Affinity purified

Immunogène: Recombinant human PKM2 protein

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 1.0 mg/mL in phosphate buffer without Mg2+/Ca2+, pH 7.4, 150 mM NaCl and 50 % glycerol

Agent conservateur: Without preservative

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur PKM

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Autre désignation: PKM

Sujet:

Background: Pyruvate kinase (PK) regulates the final rate-limiting step of glycolysis in the production of pyruvate and adenosine triphosphate (ATP). Alternate splicing of PKM pre-mRNA leads to PKM2 generation by the inclusion of exon 10 and the exclusion of exon 9, which is specific for PKM1. Besides its cytosolic roles in glycolysis, PKM2, which is upregulated by growth factor receptor activation 1, is phosphorylated at S37 by extracellular signal-regulated kinase (ERK) 2. This phosphorylation leads to the cis-trans isomerization of PKM2 by the peptidyl-prolineisomerase protein interacting with never in mitosis A 1 (PIN1), exposure of the nuclear localization signal (NLS) of PKM2, and subsequent binding of importin α 5 for nuclear translocation 2. In the nucleus, PKM2binds to phosphorylated Y333 of β-catenin, which is essential for β-catenin transactivation 3, and interacts with and phosphorylates histone H3 at T11, leading to H3-K9 acetylation and transcription of genes such as MYC and CCND14. c-Myc expression results in the upregulation of GLUT1, lactate dehydrogenase A (LDHA), and, in a positive feedback loop, PTB-dependent PKM2, which subsequently enhances the Warburg effect 2. Cyclin D1 expression, in turn, promotes G1-S phase transition 3, 4.1 Yang W, Xia Y, Cao Y et al. EGFR-induced and PKCepsilonmonoubiquitylation-dependent NF-kappaB activation upregulates PKM2 expression and promotes tumorigenesis. Molecular cell 2012; 48:771-784.2 Yang W, Zheng Y, Xia Y et al. ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect. Nature cell biology 2012; 14:1295-1304.3 Yang W, Xia Y, Ji H et al. Nuclear PKM2 regulates beta-catenin transactivation upon EGFR activation. Nature 2011; 480:118-122.4 Yang W, Xia Y, Hawke D et al. PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis. Cell 2012; 150:685-696.

Aliases: PKM; PK3; OIP3; PK2;

UniProt: P14618

Pathways: L'effet Warburg