XRCC6 anticorps

N° du produit ABIN7181844

L’anticorps Lapin Polyclonal anti-XRCC6 a été validé pour WB, IHC, ELISA et IF. Il convient pour détecter XRCC6 dans des échantillons de Humain.

Aperçu rapide pour XRCC6 anticorps (ABIN7181844)

Antigène: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp XRCC6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-XRCC6 anticorps

Fonction: XRCC6/XRCC5 Antibody

Réactivité croisée: Souris, Rat

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from Human Ku70/80.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur XRCC6

Antigène: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Autre désignation: XRCC6

Sujet:

Background: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

Shankar Mitra, AAPG Bulletin, Oct 2006, 90: 1565 - 1584.
Ward Giffin, Mol. Cell. Biol., Jun 1999, 19: 4065 - 4078.
Huichen Wang, Nucleic Acids Res., Sep 2003, 31: 5377 - 5388.
Boris Kysela, J. Biol. Chem., Jun 2003, 278: 22466 - 22474.

Aliases: 5''-deoxyribose-5-phosphate lyase Ku70 antibody, 5''-dRP lyase Ku70 antibody, 70 kDa subunit of Ku antigen antibody, ATP dependent DNA helicase 2 subunit 1 antibody, ATP dependent DNA helicase II 70 kDa subunit antibody, ATP-dependent DNA helicase 2 subunit 1 antibody, ATP-dependent DNA helicase II 70 kDa subunit antibody, CTC box binding factor 75 kDa subunit antibody, CTC box-binding factor 75 kDa subunit antibody, CTC75 antibody, CTCBF antibody, DNA repair protein XRCC6 antibody, G22P1 antibody, Ku 70 antibody, Ku autoantigen p70 subunit antibody, Ku autoantigen, 70 kDa antibody, Ku p70 antibody, Ku70 antibody, Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody, Kup70 antibody, Lupus Ku autoantigen protein p70 antibody, ML8 antibody, Thyroid autoantigen 70kD (Ku antigen) antibody, Thyroid autoantigen antibody, Thyroid lupus autoantigen antibody, Thyroid lupus autoantigen p70 antibody, Thyroid-lupus autoantigen antibody, TLAA antibody, X ray repair complementing defective repair in Chinese hamster cells 6 antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 antibody, X-ray repair cross-complementing protein 6 antibody, XRCC 6 antibody, Xrcc6 antibody, XRCC6_HUMAN antibody

UniProt: P12956

Pathways: Réparation de l'ADN