MSH2 anticorps

N° du produit ABIN7183857

L’anticorps Lapin Polyclonal anti-MSH2 a été validé pour WB, ELISA, IHC et IF. Il convient pour détecter MSH2 dans des échantillons de Humain.

Aperçu rapide pour MSH2 anticorps (ABIN7183857)

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MSH2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-MSH2 anticorps

Fonction: MSH2 Antibody

Réactivité croisée: Souris

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from internal of Human MSH2.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur MSH2

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Autre désignation: MSH2

Sujet:

Background: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Fishel R., Cell 75:1027-1038(1993).
Wang Y., Genes Dev. 14:927-939(2000).
Wang Y., Proc. Natl. Acad. Sci. U.S.A. 100:15387-15392(2003).

Aliases: BAT26 antibody, COCA 1 antibody, COCA1 antibody, DNA mismatch repair protein Msh2 antibody, FCC 1 antibody, FCC1 antibody, hMSH2 antibody, HNPCC 1 antibody, HNPCC antibody, HNPCC1 antibody, LCFS2 antibody, MSH 2 antibody, Msh2 antibody, MSH2_HUMAN antibody, MutS homolog 2 antibody, MutS homolog 2 colon cancer nonpolyposis type 1 antibody, MutS protein homolog 2 antibody

UniProt: P43246

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response