14-3-3 theta anticorps (pSer232)

N° du produit ABIN7223210

L’anticorps anti-14-3-3 theta Polyclonal Lapin est utilisé pour la détection de 14-3-3 theta dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour 14-3-3 theta anticorps (pSer232) (ABIN7223210)

Antigène: 14-3-3 theta (YWHAQ)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp 14-3-3 theta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-14-3-3 theta anticorps

Épitope: pSer232

Fonction: Rabbit Anti-14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody

Specificité: Phospho-14-3-3 θ/τ (S232) Polyclonal Antibody detects endogenous levels of 14-3-3 θ/τ protein only when phosphorylated at S232.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human 14-3-3 theta/tau Phospho-Ser232

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur 14-3-3 theta

Antigène: 14-3-3 theta (YWHAQ)

Autre désignation: 14-3-3 theta/tau

Sujet: YWHAQ, 14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1The protein encoded by PLN (phospholamban) is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in PLN are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.

Poids moléculaire: 28kD

ID gène: 5350

UniProt: P27348

Pathways: Apoptose, Myometrial Relaxation and Contraction