alpha Actinin anticorps

N° du produit ABIN7212180

L’anticorps anti-alpha Actinin Monoclonal Souris est utilisé pour la détection de alpha Actinin dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour alpha Actinin anticorps (ABIN7212180)

Antigène: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Actinin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 4A12

Détail du produit anti-alpha Actinin anticorps

Fonction: Mouse Anti-a-actinin Monoclonal Antibody (4A12)

Specificité: A-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogène: Recombinant Protein of a-actinin of ACTN1

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:200-400,IF/ICC1:200-400,WB 1:500-2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur alpha Actinin

Antigène: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Autre désignation: a-actinin

Sujet: ACTN1Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN1 (actinin alpha 1) encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for ACTN1.

Poids moléculaire: 103kD

ID gène: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization, Negative Regulation of Transporter Activity